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. 2013 Oct;27(10):4184–4193. doi: 10.1096/fj.13-228486

Figure 1.

Figure 1.

Tonic, oxidative muscles have higher autophagy flux, autophagy, and mitophagy protein expression than phasic, glycolytic muscles. Immunoblot analyses were performed for homogenates of tonic, oxidative soleus (SO), plantaris (PL) with mixed fiber types, and phasic, glycolytic white vastus lateralis (WV) muscles. A) Representative immunoblot images of LC3, p62, Atg7, Atg6, Bnip3, Pgc-1α, Cyc, Cox4, and β-actin. B) Quantification of LC3-II, LC3-II/LC3-I ratio, and p62 protein collectively as an index of autophagy flux. C) Quantification of LC3 (LC3-I+LC3-II), Atg7, and Atg6 proteins. D) Quantification of the mitophagy protein Bnip3. E) Quantification of Pgc-1α, Cyc, and Cox4 proteins as indexes of mitochondrial biogenesis. Protein expression comparisons were performed after normalization to β-actin. Results are represented as means ± se (n=4/muscle). *P < 0.05 vs. WV; #P < 0.05 vs. PL.