Figure 3.

Exercise training promotes autophagy expression, but not basal autophagy and mitophagy protein expression in tonic, oxidative muscle. Immunoblot analyses were performed for homogenates of soleus muscle from mice that underwent 4 wk of voluntary wheel-running exercise (EX-4 wk) compared to the sedentary control (SED). A) Representative immunoblot images of LC3, p62, Atg7, Atg6, Bnip3, Pgc-1α, Cyc, Cox4, and Gapdh in sedentary and trained mice. B) Quantification of LC3-II, LC3-II/LC3-I ratio, and p62 protein collectively as an index of autophagy flux. C) Quantification of LC3 (LC3-I+LC3-II), Atg7, and Atg6 proteins. D) Quantification of the mitophagy protein Bnip3. E) Quantification of Pgc-1α, Cyc, and Cox4 proteins as indexes of mitochondrial biogenesis. Protein expression comparisons were performed after normalization to Gapdh. Results are represented as means ± se (n=10/group). *P < 0.05, ***P < 0.001.