TABLE 2.
Characteristics of assays
| Characteristic | Multi-Test Dip-S-Ticks | TyphiDot | TUBEX | Widal |
|---|---|---|---|---|
| Approximate cost (U.S. dollars)/specimena | 10 | 2.14 | 4.00 | 0.50 |
| No. of tests/kit | 50 | 56 | 30 | 55 |
| Antibody | IgM and IgG | IgM or IgG | IgM | IgM and IgG |
| Antigen | O, H, and Vi | OMPb | O9 | O, H, and Vi |
| Amt of serum needed (μl) | 10 | 2.5 | 35-40 ± (one drop) | 300 ± (two dilutions) |
| Reaction time (min) | 90 | 60 | 3 | 5c |
| Temp for storage (°C) | 2-8 | 2-8 | 2-8 | 2-8 |
a
Personal communication (Prue Griffin, PANBIO INDX, Inc.; Kok-hai Ong, Malaysian Bio-Diagnostic Research Sdn. Bhd.; Helena Goike, IDL Biotech). TyphiDot price includes running each sample twice; once for IgM and once for IgG (an additional $2.93 should be added for each TyphiDot-M run on IgM-negative, IgG-positive specimens). All costs assume samples are batched to maximize kit use; use of the kits for single use increases the price per specimen. Costs do not include shipping.
b
OMP, outer membrane protein.
c
As determined by the rapid centrifugation technique (the classical technique is 2 h to overnight).