FIG. 1.

Vitamin C increases the proliferation and survival of adipose-derived stem cells (ASCs). We compared the stimulatory effects of vitamin C in vitro, and vitamin C increased the proliferation and survival of ASCs. (A) Cell proliferation was measured by the CCK-8 assay, and vitamin C increased the proliferation of ASCs in a dose-dependent manner. (B) The mRNA expression of pluripotency markers was measured by quantitative real-time polymerase chain reaction (QPCR), and vitamin C significantly induced the expression of Oct4, Sox2, and Nanog. (C, D) Cell damage was induced by hydrogen peroxide (H₂O₂) treatment (500 μM, C) and UVB irradiation (500 mJ/cm², D) of ASCs, and vitamin C (200 μM) significantly increased the survival of ASCs, as determined by the CCK-8 assay. (E) Vitamin C attenuated the H₂O₂-induced sub-G1 of ASCs as measured by flow cytometry. (F) Reactive oxygen species generation was measured using 2′,7′-dichlorofluorescin diacetate, and vitamin C reduced the fluorescence intensity, as determined by flow cytometry. Data are mean±STD (**P<0.01, Student's t-test). Color images available online at www.liebertpub.com/scd