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. 2014 Feb 13;23(12):1364–1376. doi: 10.1089/scd.2013.0460

FIG. 6.

FIG. 6.

Vitamin C upregulates growth factor expression. The expression of diverse growth factors was induced by vitamin C treatment, and they collectively enhanced the hair growth promoting effect of ASCs. (A) In a growth factor antibody array, the expression of hepatocyte growth factor (HGF) and insulin-like growth factor binding protein 6 (IGFBP6) protein was increased by vitamin C treatment. (B) QPCR showed that the expression of HGF mRNA was significantly induced at 4 and 24 h after vitamin C (200 μM) treatment. (C) IGFBP6 mRNA was slightly upregulated at 24 h. (D) Vitamin C treatment induced HGF mRNA expression in a dose-dependent manner at 24 h. (E) In addition, an ELISA measure of HGF protein levels in CM showed that HGF secretion was ∼30% higher in vit.C-CM than in ASC-CM. (F) mRNA expression of VEGF, bFGF, and KGF increased significantly after 4 h of vitamin C treatment. Data are mean±STD (**P<0.01, Student's t-test).