FIG. 2.

Induction of putative human corneal endothelial cells (hCECs) from POMPs. (A) Morphological changes of POMPs after exposure to the CEC differentiation medium (EM) for further induction over 2 weeks. (B) Immunostaining of the plated EBs and POMPs after 14 days of exposure to EM. Coexpression of N-cadherin and vimentin was found in putative hCECs. The pictures at the bottom are higher magnifications of differentiated POMPs. (C) Analysis of N-cadherin/vimentin dual-positive cells by FACS. The mean proportion of dual-positive cells was about 3.56% 7 days after induction and increased to 7.31% on day 14, whereas there was no increase from 14 to 21 days. The dot plots are representative of three independent experiments. Scale bars=20 μm.