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. 2014 Feb 5;23(12):1340–1354. doi: 10.1089/scd.2013.0510

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 5. — Surgical procedure for the CEC-like cell sheet transplantation. (A) A diameter of 7.5-mm circle trace marker was made on the central rabbit corneal surface using a trephine. (B, C) The Descemet's membrane was spitted in circumference along the circle trace with a sharp hook (B) and separated from the stroma bluntly (C). (D) The isolated Descemet's membrane was removed from the anterior chamber of the eye. (E) The surface of Descemet's membrane of the CEC-like cell sheet was coated with an ophthalmic viscosurgical device. (F) The sheet was folded and inserted into the anterior chamber. (G) The folded sheet was spread out in the anterior chamber. (H) The sheet was attached to the posterior stroma by injecting a sterile air bubble into the anterior chamber.