FIG. 4.

Stromal cultured LTC-ICs exhibit engraftment potential in NSG mice. Bulk cell populations were harvested from each LTC-IC condition after 5 weeks of culture. Cells were then divided among two to four nonlethally irradiated NSG mice and injected through the tail vain at a dose equivalent of 7.5–10×10³ CD34⁺ UCB initially seeded per mouse. Each group had at least one male and at least one female and was divided evenly where possible. (A) Flow cytometric analysis of peripheral blood, spleen, and bone marrow from a single mouse representing each experimental group. (B) Dot plot representing % human CD45 chimerism in each tissue for individual mice. Horizontal bars represent the group mean values. BM, bone marrow; PB, peripheral blood; N.A., nothing applied (no cells injected).