FIG. 2.

HIV-specific LAG-3⁺CD8⁺ T cells express a variable surface immunophenotype. PBMCs were stimulated with HIV peptides then stained with Vivid for live/dead cell exclusion, LAG-3 FITC, CTLA-4 PE-Cy5, CD3 AmCyan, CD8 APC H7, CD25 PE, CD127 Alexa Fluor 647, and CD45RA PE-Cy7 and analyzed by flow cytometry. Samples were first gated on SSC/FSC, followed by SSC/CD3⁺, and then on the CD3⁺/CD8⁺ lymphocyte population; the percent of LAG-3⁺CD8⁺ T cells was then determined. (A) Two representative plots of HIV-specific LAG-3⁺CD8⁺ T cells after Gag B peptide stimulations that express CD25⁺ and are CD127 low. (B) HIV-specific LAG-3⁺CD8⁺ T cells with variable expression of CD45RA and CCR7. Box inserts within each quadrant represent the indicated cell subpopulations. Dotted arrows indicate further subgating of indicated cell subpopulations.