FIG. 3.

LAG-3⁺CD8⁺ T cell populations are distinct from PD1⁺, CTLA4⁺, or 2B4⁺ CD8⁺ T cells. PBMCs were stimulated with HIV-specific peptide Gag B peptides then stained with Vivid for live/dead cell exclusion, LAG-3 FITC, 2B4 PE, CD3 AmCyan, PD-1 APC, CTLA-4 PE-Cy5, CD4 PE-Cy7, and CD8 APC-H7. Samples were first gated on the CD3⁺/CD8⁺ lymphocyte population; the percent of LAG3⁺, PD-1⁺ CTLA4⁺, or 2B4⁺ cells was then determined. Representative plots of the frequencies of the LAG-3⁺ Gag-specific CD8⁺ T cells and expression of PD-1⁺ CTLA4⁺ or 2B4⁺ are shown. Responses ≥0.1% and two times the background were considered positive.