TABLE 5.
Investigation of different amounts of purified VAR DNA by LightCycler PCR combined with melting-curve analysis
| Amt of viral DNA (input [no. of genome copies) | Avg Ct/avg Tm (°C)a
|
||
|---|---|---|---|
| Strain Bangladesh | Bangladesh spiked with 10 ng of human genomic DNA/reaction | Strain Variolator 4 | |
| 1 ng (5.7 × 106) | 15.49/57.21 | 15.63/56.68 | 15.09/56.74 |
| 100 pg (5.7 × 105) | 18.89/57.24 | 18.91/56.73 | 18.52/56.85 |
| 10 pg (5.7 × 104) | 22.50/57.35 | 22.48/56.98 | 21.91/57.02 |
| 1 pg (5.7 × 103) | 25.80/57.44 | 25.92/57.02 | 25.49/57.14 |
| 100 fg (5.7 × 102) | 29.06/57.55 | 29.34/57.23 | 28.88/57.29 |
| 10 fg (5.7 × 101) | 32.71/57.80 | 32.49/57.55 | 31.87/57.51 |
| 1 fg (5.7) | 35.43/58.11 | 35.22/57.99 | 34.91/58.07 |
a
Ct, the average cycle at which the fluorescence crossed the threshold. Values represent the means of triplicate reactions.