Fig. 3.

Chronic effects of S-LPS and R595 on pacemaker current of human atrial myocytes.

Cells were incubated with S-LPS or R595 (10 μg/mL, 6–10 h. (A) Mean I–V relationships of I_f in control and endotoxin-treated cells. (B) I_f steady-state activation for control and R595-treated cells. Normalized conductances were fitted by a Boltzmann function. Control: V_1/2 = − 83.3 ± 1.2 mV, k = 7.8 ± 0.4 mV; R595: V_1/2 = − 80.3 ± 1.3 mV, k = 7.0 ± 0.5 mV. (C) Mean time constants of I_f activation for control and R595-treated cells. Values were obtained by fitting a monoexponential function to current traces. ⁺p ≤ 0.05 vs. control and R595; ^§p ≤ 0.05 vs. R595.