Table 4.

Nucleotide diversity (π) in different regions of tRNAs between Nasonia and honey bee.

tRNA_location	Conserved+/+	Conserved+/−	Moderately_Conserved&	Least_Conserved#
Acceptor_Arm	H,F	N,C,W,E,I	V,D,Q,K,P,M,A,T	R,G,L,S
D_Arm	N,C,E,Q,H,I,L,K,F,W	D,M,P,A,V	R,S,T	G
T_Arm	D,C,CQ,H,P,W	N,G,E,F,I,L,S	A,V,M,K,R	T
Anticodon_Loop	N,D,C,H,I,F,W	none	M,Q,K,E,G,T,P,A	R,V,S,L
A-box	C,E,H,W	P,N,D,Q,F,A,G	I,V,K,M,R,L,T,S	none
B-box	C,E,H,W	P,N,D,Q	F,I,V,K,A,M,R,L,T	S

Note: +/+ means that each of these are perfectly conserved in both Nvit and Amel; +/− means that these genes are conserved in one species but show variation (π values within 0 to 0.2) in the other; the symbol ‘&’ means that genes show moderate levels of sequence variation (π values within 0 to 0.2); the symbol ‘#’ means that these genes show higher sequence variation (π > 0.2) suggesting that these are least conserved among tRNA genes of these two species. The entries with single letter abbreviations represent the standard amino acids to which the tRNA gene is cognate.