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. 2011 Sep;18(9):832–841. doi: 10.1177/1933719111398501

Figure 2.

Figure 2.

The effects of AMG 479 on insulin growth factor-1 receptor (IGF-1-R) activity. RL-95-2 and ECC-1/PRAB72 cells were starved overnight and then treated with 5% fetal bovine serum (FBS) and varying concentrations of AMG 479 alone for 60 minutes (A), or treated with AMG (2 nmol/L) in combination with IGF-1 for 15 minutes (B). The phosphotyrosine levels (Tyr 1131) of the activated IGF-1-R were measured by enzyme-linked immunosorbent assay (ELISA). Treatment with AMG 479 alone significantly reduced IGF-1-R activity in a dose-dependent manner in both of the endometrial cancer cell lines (A). As expected, cells treated with IGF-1 alone (3.7 nmol/L) for 15 minutes demonstrated a dramatic increase in IGF-1-R kinase activity (B). AMG 479 (2 nmol/L) was able to potently block IGF-1 (0.15-7.5 nmol/L) stimulated autophosphorylation of the IGF-1-R in both endometrial cancer cell lines (B). These results are representative of two2 independent experiments (* Indicates statistically significant difference.).