Figure 3.

Induction of cell-cycle arrest by AMG 479. RL-95-2 and ECC-1/PRAB72 cells were cultured for 24 hours and treated with AMG 479 at varying concentrations in 5% fetal bovine serum (FBS) for 36 hours (A and B) or starved overnight and then stimulated with 15% serum and AMG 479 at the noted concentrations for 36 hours (C and D). Cell-cycle analysis was performed by flow cytometry. AMG 479 inhibited cell-cycle progression by arrest in G1 phase in the ECC-1/PRAB72 cells, and G2/M phase in the RL-95-2 cells. Results shown are representative of 1 of the 3 independent experiments. Statistical significant results are indicated by a *, as determined using the Student t test.