Figure 4.

The effects of AMG 479 on apoptosis. The RL-95-2 and ECC-1/PRAB72 cells were cultured for 24 hours and then treated with AMG 479 at the indicated concentrations for an additional 24 hours. Apoptosis was assessed using an antibody to caspase-3. AMG 479 increased apoptosis in the ECC-1/PRAB72 cell line but only at high doses of treatment (200 nmol/L). Lower doses of AMG 479 had little effect on caspase-3 activity in this cell line. For the RL-95-2 cell line, there was no significant increase in caspase-3 activity at any dose of AMG 479. The results are shown as the mean ± SD and are representative of 2 independent experiments (* indicates statistically significant difference.).