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Relative expression of ZEB1 (A), ZEB2 (B), and CDH1 (C), in Ishikawa cells transfected with pre-miR-200c or pre-miR negative control (NC) determined by real-time polymerase chain reaction (PCR). The results are presented as mean ± standard error of the mean (SEM) and analyzed using nonparametric student t test with P values indicated by corresponding lines.
