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The effect of gain of function of miR-200c on Ishikawa cell proliferation. The cells were transfected with pre-miR-200c or pre-miR negative control (NC) and cell proliferation was determined using MTS assay on indicated days. The results are shown as mean ± standard error of the mean (SEM) and analyzed using nonparametric student t test (*P < .05 as independently compared to corresponding negative control for each day).
