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. 2012 Sep;19(9):911–922. doi: 10.1177/1933719112446086

Figure 2.

Figure 2.

A, Percentage of spermatogenic cell types in testis from D6 to D88 mouse testis. The age of the mice and counted spermatogenic cell types are specified for each fraction. B, Isolation of spermatogenic cells by fractionation of mixed germ cells from D6 to D88 mouse testis in a 2% to 4% bovine serum albumin (BSA) gradient. Photomicrographs of air-dried and hematoxylin–eosin-stained smears of spermatogenic cells isolated from immature or mature testis. The images were taken at an original magnification of ×100; inserts were at ×1000 magnification. C, Percentage of spermatogenic cell types in fractions obtained from D6 to D88 mouse testis by separation of mixed germ cells using a 2% to 4% BSA gradient. One hundred hematoxylin and eosin (HE)-stained cells were assessed for each fraction. The age of the mice and isolated spermatogenic cell types are specified for each fraction. D, Expression of Scp3 (synaptonemal complex 3) and Prm2 (protamine 2) in isolated SC and RS fractions. Scp3, a gene expressed specifically in spermatocytes, was detected in the SC fraction, but not in the RS fraction. Prm2, a gene expressed specifically in round spermatids, was detected in the RS fraction, but not in the SC fraction, suggesting efficient isolation. β-Actin was used as an endogenous control. SG indicates spermatogonia; SC, spermatocytes; RS, round spermatids; ES, elongated spermatids; RB, residual bodies; OC, other cells.