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. 2012 Sep;19(9):911–922. doi: 10.1177/1933719112446086

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© The Author(s) 2012

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Figure 3. — Quantification of Epab messenger RNA (mRNA) expression in isolated spermatogenic cells from immature and mature mouse testes. Qualitative real-time polymerase chain reaction (qRT-PCR) in all samples was normalized to β-actin expression used as an internal control. In spermatogonia, Epab mRNA expression was low on D6, D8, and D16 (immature mice), and reached the highest value at D32. In spermatocytes Epab expression reached its highest level at D32. Epab mRNA expression in round spermatids isolated from 32- (immature) or 88-day-old (mature) mice was high, but there was no remarkable change between the 2 time points. SG indicates spermatogonia; SC, spermatocyte; RS, round spermatid; ES, elongated spermatid. SG: *P < .05 for D32 versus D16, D8, or D6; SC: *P < .05 for D32 versus D29; **P < .05 D88 and D32.