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. 2012 Sep;19(9):911–922. doi: 10.1177/1933719112446086

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Figure 4. — Quantification of Pabpc1 messenger RNA (mRNA) expression in isolated spermatogenic cells from immature and mature mouse testes. Qualitative real-time polymerase chain reaction (qRT-PCR) was normalized to β-actin expression used as an internal control. Pabpc1 expression began to rise at D8 and reached the highest value at D32. Pabpc1 mRNA expression reached its highest level at D32. In round spermatid fractions isolated from 32- (immature) and 88-day-old (mature) mice there was no remarkable change in Pabpc1 expression. SG indicates spermatogonia; SC, spermatocyte; RS, round spermatid; ES, elongated spermatid. SG: *P < .05 for D16 versus D8; **P < .05 for D32 versus D16; SC: *P < .05 for D32 versus D88 and D29.