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. 2012 Sep;19(9):911–922. doi: 10.1177/1933719112446086

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© The Author(s) 2012

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Figure 5. — Quantification of Epab and Pabpc1 messenger RNA (mRNA) expression in isolated spermatogenic cells from mature male mouse using Qualitative real-time polymerase chain reaction (qRT-PCR). Spermatocytes, round spermatids, and elongated spermatids were isolated from mature male testis, and spermatozoa were isolated from cauda epididymis. The qRT-PCRs for Epab and Pabpc1 were normalized to β-actin expression used as an internal control. A, Comparable with RNA in situ hybridization (ISH), round spermatids showed significantly higher Epab expression than spermatocytes, elongated spermatids, and spermatozoa. *P < .05 RS versus SC, ES, and spermatozoa. B, Comparable with RNA ISH, Pabpc1 expression in spermatocytes, and round spermatids was higher than elongated spermatids and spermatozoa. Moreover, qRT-PCR revealed that SC fractions had significantly more Pabpc1 mRNA than RS. SC indicates spermatocyte; RS, round spermatid; ES, elongated spermatid. *P < .05 RS versus SC; **P < .05 ES and spermatozoa versus RS.