Figure 6.

Localization of Epab and Pabpc1 messenger RNA (mRNA) by in situ hybridization in mature male mouse testis. A, Epab expression in mature male mouse testis. Epab expression was assessed by in situ hybridization using a Epab exon 2 probe. Epab expression was highest in spermatogonia (arrows) localized at the periphery of the tubules. In addition, RSs had higher expression compared with spermatocytes or elongated spermatids. B, Pabpc1 expression in mature male mouse testis. Pabpc1 expression was assessed by in situ hybridization using a Pabpc1 exon 1-2 probe. Pabpc1 expression was cytoplasmic and was localized to the seminiferous tubules as well as the intertubular areas. In seminiferous tubules, spermatocytes and RSs had higher Pabpc1 expression than spermatogonia or elongated spermatids. C, Negative control for in situ hybridization. D, Hematoxylin and eosin stained mature male mouse testis. L indicates lumen of the seminiferous tubule; ES, elongated spermatid; RS, round spermatid; SC, spermatocyte; IT, intertubular area.