Figure 7.

Localization of Epab messenger RNA (mRNA) by in situ hybridization in postnatal male mouse testis from D6 to D32. Hematoxylin–eosin staining was also done for each stage. Epab expression was assessed by in situ hybridization using a Epab exon 2 probe. While Epab expression in spermatogonia was very weak on D6, D8, and D16, it increased significantly by D29 and D32. Spermatocytes had slightly higher Epab expression in D16 than D29 or D32 testes. In RSs, Epab expression in D32 testis was found to be more than D29 testis. L indicates lumen of the seminiferous tubule; ES, elongated spermatid; RSs, round spermatid; SC, spermatocyte; IT, intertubular area.