A. Resistant (A427-R and DV-90-R) and parental cells were treated with 1 μM AZD6244/GDC-0941 and cell proliferation measured.
B. A427 and A427-R cells were treated with 1 μM AZD6244/GDC-0941 (AG) or vehicle (V) for 24 hours. Lower: Quantitation of protein expression from western blots. Data are mean and error of three independent experiments.
C. Cells were treated with 1 μM AZD6244/GDC-0941 for 24 hours, stained with propidium iodide and cell cycle populations analyzed by flow cytometry.
D. Cells were treated with 1 μM AZD6244/GDC-0941 and apoptosis determined by annexin staining. Data shown are mean and standard error of 5-7 independent experiments.