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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cancer Res. 2014 Mar 27;74(11):3146–3156. doi: 10.1158/0008-5472.CAN-13-3728

Figure 4. Loss of apoptotic response underlies adaptive resistance to MEKi/PI3Ki in vitro.

Figure 4

A. Resistant (A427-R and DV-90-R) and parental cells were treated with 1 μM AZD6244/GDC-0941 and cell proliferation measured.

B. A427 and A427-R cells were treated with 1 μM AZD6244/GDC-0941 (AG) or vehicle (V) for 24 hours. Lower: Quantitation of protein expression from western blots. Data are mean and error of three independent experiments.

C. Cells were treated with 1 μM AZD6244/GDC-0941 for 24 hours, stained with propidium iodide and cell cycle populations analyzed by flow cytometry.

D. Cells were treated with 1 μM AZD6244/GDC-0941 and apoptosis determined by annexin staining. Data shown are mean and standard error of 5-7 independent experiments.