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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Eur J Immunol. 2013 Aug 27;43(11):3061–3075. doi: 10.1002/eji.201343416

Figure 8. Variable strength of IgM signal in J-chain positive and negative spleen cells.

Figure 8

In situ hybridization double stain of 6 μm sections spleen tissue from an adult shark (shark W). (A) Slides were double stained with IgM-C3-C4-DIG (left) and J-chain-FLU (middle) riboprobes. Images merged at right. Photographs taken at 40× magnification and marked with a 100 μm scale bar. (B) Data from 50 cells each of J-chain+ and J-chain- cells (from multiple fields of view) were pooled to get an average mean fluorescent intensity of the IgM stain (green). (*) indicates p-value of less than 0.05 (T-test). Error bars signify SEM.

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