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. 2004 May;42(5):1982–1985. doi: 10.1128/JCM.42.5.1982-1985.2004

FIG. 1.

FIG. 1.

Demonstration of coccidioidal PCR products after agarose gel electrophoresis. A 100-bp DNA ladder was used to estimate the product sizes (lane 1). (A and B) Coccidioidal genomic DNAs were added to PCRs in the following quantities to determine the sensitivity of detetion. Lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg; lane 8, no DNA added. Gels were stained with ethidium bromide (A) or Vistra Green (B). (C) Recovery of coccidioidal DNA added to serum. Lane 2, coccidioidal DNA added to normal human serum after the isolation procedure and then amplified by PCR; lane 3, coccidioidal genomic DNA added to normal human serum prior to isolation and PCR amplification; lane 4, negative (no template) control reaction. The gel was stained with ethidium bromide.