TABLE 1.
Precisions of the Roche and Artus assays for detection of SARS CoVa
| Nominal concn (no. of copies/ml) | Roche assay
|
Artus assay
|
||||||
|---|---|---|---|---|---|---|---|---|
| Mean measured concn (no. of copies/ml [range]) | Mean Ct | SD | % CV | Mean measured concn (no. of copies/ml [range]) | Mean Ct | SD | % CV | |
| 106 | 4,974,250 (3,686,000-5,992,000) | 26.38 | 0.22 | 0.82 | 3,401,750 (2,531,000-4,409,000) | 25.52 | 0.21 | 0.83 |
| 105 | 514,925 (340,000-689,000) | 29.58 | 0.25 | 0.84 | 301,950 (222,800-365,500) | 28.86 | 0.19 | 0.66 |
| 104 | 76,688 (52,080-107,720) | 32.28 | 0.37 | 1.15 | 30,684 (23,740-37,480) | 32.00 | 0.18 | 0.55 |
| 103 | 22,057 (14,586-28,540) | 33.98 | 0.15 | 0.45 | 3,716 (2,822-5,763) | 34.92 | 0.25 | 0.71 |
| 102 | 13,661 (8,584-20,520) | 34.73 | 0.22 | 0.62 | 439 (256-766) | 37.95 | 0.57 | 1.49 |
| 101 | 17,460 | 34.33 | 220 (85-165) | 39.14 | 0.88 | 2.24 | ||
a
External standard RNAs (a gamma-irradiated, purified Vero cell culture supernatant was used as a full-virus quantification standard) were tested at six different concentrations. Each concentration was analyzed in eight PCR runs. Ct represents the PCR cycle at which the probe-specific fluorescent signal can be detected against the background.