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Sensitivity of detection of C. albicans ACT1 mRNA by RT-PCR using either random hexamer primers (lanes 2 to 5) or a gene-specific reverse primer (lanes 6 to 9) for cDNA preparation. PCR amplification was performed on cDNA prepared from 10 ng (lanes 2 and 6), 1 ng (lanes 3 and 7), 0.1 ng (lanes 4 and 8), and 10 pg (lanes 5 and 9) of C. albicans total RNA. Lane 1, negative-control PCR (no cDNA added). Lane M: DNA molecular size markers (bp).
