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. 2004 May;42(5):2275–2278. doi: 10.1128/JCM.42.5.2275-2278.2004

FIG. 2.

FIG. 2.

Use of multiplex RT for simultaneous preparation of gene-specific cDNAs in a single reaction. The RT reaction used a mixture of C. albicans and mouse (1:100) total RNA and the reverse primers for C. albicans 18S, ACT1, EFB1, SAP2, ADH1, and ADH2 transcripts. The cDNA template was used in individual PCRs with the primer pairs for the 18S (lane 2), ACT1 (lane 5), EFB1 (lane 7), SAP2 (lane 9), ADH1 (lane 12), and ADH2 (lane 15) gene transcripts. Positive PCR control experiments were performed using either 0.1 ng of C. albicans cDNA preparations (ACT1 and EFB1 [lanes 4 and 6, respectively]) or 0.1 ng of C. albicans genomic DNA (18S, SAP2, ADH1, and ADH2 [lanes 1, 8, 11, and 14, respectively]) as a template. As a control for contamination by C. albicans genomic DNA, a mock-RT reaction (without enzyme) was performed and the reaction mixture was used in PCRs with the 18S (lane 3), SAP2 (lane 10), ADH1 (lane 13), and ADH2 (lane 16) primer pairs. Lane M: DNA molecular size markers (bp).