Skip to main content
. 2004 May;42(5):2275–2278. doi: 10.1128/JCM.42.5.2275-2278.2004

FIG. 3.

FIG. 3.

Detection of single-copy C. albicans gene mRNAs in FFPE human archival biopsies. PCR amplification was performed using primer pairs for the ACT1 (A), EFB1 (B), SAP2 (C), ADH1 (D), and ADH2 (E) genes. Template cDNA was prepared by multiplex RT with RNA extracted from FFPE samples of CHC biopsies (lanes 4 to 8), PC biopsies (lanes 9 to 10), and a periapical granuloma biopsy (lane 3) as a negative control. Lane 1, negative control of PCR without cDNA template; lane 2, positive control of PCR using either 0.1 ng of C. albicans cDNA (ACT1 and EFB1) or 0.1 ng of C. albicans genomic DNA (SAP2, ADH1, and ADH2). Lane M: DNA molecular weight markers.