TABLE 1.

Primer pairs used to detect C. albicans RNA transcripts by RT-PCR

Gene	Accession no.	PCR forward primer sequence	PCR reverse and RT primer sequence	PCR product size(s) (bp)	Annealing temp (°C)
18S rRNA	AF114470	GGATTTACTGAAGACTAACTACTG	GAACAACAACCGATCCCTAGT	131	60
ACT1	X16377	ATGGACGGGAAGAAGTTGC	CCCATACCAACCATGATACC	146	62
EFB1a	X96517	TCAGATTTCTCTAAAGTCG	TGACATCAGCTTGAGTGG	97 and 462	58
SAP2b	M83663	AACAACAACCCACTAGACATCACC	TGACCATTAGTAACTGGGAATGCTTTAGGA	178	60
ADH1	X81694	CACTCACGATGGTTCATTCG	AAGATGGTGCGACATTGG	90	60
ADH2	AL033501	CACCCACGATGGTTCTTTCC	AAGATTGGTGCAACATGGG	90	60

^aPrimers designed on the basis of work by Schofield et al. (22).

^bPrimers designed on the basis of work by Naglik et al. (14).