Figure 5. The effect of fission factor deletions on peroxisome abundance statistics.
Budding yeast cells containing peroxisomes labeled by YFP-PTS1 and lacking one of the peroxisome fission factors VPS1, DNM1 or its accessory factor FIS1 were cultured in medium containing 0.2% oleic acid for 20 hr. Single cell peroxisome abundance distributions were measured for each of these strains. The Fano factors are plotted as a function of mean peroxisome abundance extracted from the single cell peroxisome distributions. Figure 5—figure supplement 1 shows data similar to Figure 5 but with Pex3-mRFP as the peroxisome marker.
Figure 5—figure supplement 1. Peroxisome abundance fluctuations in cells containing deletions of the fission factors Dnm1, Fis1 and Vps1 measured labeling of peroxisomes by Pex3-mRFP.
