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. 2014 Jun 5;5:252. doi: 10.3389/fpls.2014.00252

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Copyright © 2014 Matthysse.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Attachment of Agrobacterium tumefaciens strain C58 (A,C,E,G) and a UPP-deletion mutant of C58 (B,D,F,H) to quartz sand (A,B), polyester thread (C,D), yeast (Saccharomyces cerevisiae; C,F), and tomato root hairs (G,H) in a 1/10 dilution of MS medium containing a 1/20 dilution of AB minimal medium. Note the copious attachment of wild-type cells and the large decrease in attachment in a UPP deletion mutant. Approximately 10⁶ bacteria per ml were incubated with the substrate for 24 h.