TABLE 1.

Primers and probes used for RT-LightCycler PCR and Northern hybridization probe synthesis

Gene	GenBank accession no.	Primer or probed	Sequence (5′ to 3′)	PCR amplicon size (bp)
ERG11	X13296	LC forward	TGG AGA CGT GAT GCT G	204
		LC reverse	AGT ATG TTG ACC ACC CAT AA
		Donor probea	AAT CTC TGC TAC TTA TAT GAA AGA AAT TAA ACT GAG
		Acceptor probeb	GAG AAC GTG GTG ATA TTG ATC CAA AT
		NH forwardc	ATT GGT ATT CTT ATG GGT GGT CAA CAT AC
		NH reversec	CCC AAT ACA TCT ATG TCT ACC ACC ACC
CDR1	X77589	Forward primer	AAG AGA ACC ATT ACC AGG	286
		Reverse primer	AGG AAT CGA CGG ATC AC
		Donor probe	CAA GAC CAG CAT CTC CAT ATA CTG TAT
		Acceptor probe	ATT CTT TAT GCA AGT GAG GTA TGG TG
		NH forward	GAG ATC TAC CCT TTA AGA TA
		NH reverse	GAA TCG GGA TTC AAT TG
CDR2	U63812	Forward primer	ATG CTG ATG CCC TAG T	364
		Reverse primer	GCT TCC TTA GGA CAT GG
		Donor probe	ATT GTT GTT ATT CTT AGA TGA ACC AAC TTC AG
		Acceptor probe	TTA GAT TCT CAA ACT GCC TGG TCG
		NH forward	GGT ATA TAA ACT GGA CAA C
		NH reverse	GAA TCT GGG TCT AAT TGT
MDR1	X53823	Forward primer	GGA GTT TAG GTG CTG T	201
		Reverse primer	CGG TGA TGG CTC TCA A
		Donor probe	GCC AGT TGG AGA TGG ACT
		Acceptor probe	TTG GTT CAT GTG TAT CAT TTC TG
		NH forwardc	GAG TCG TAG CTA CAT TGC CAT TAA CA
		NH reversec	GGT GAT TTC TAA TGG TCT CCA TAA TGT
ACT1	X16377	Forward primer	CCA GCT TTC TAC GTT TCC	209
		Reverse primer	CTG TAA CCA CGT TCA GAC
		Donor probe	CGG TAT TGT TTT GGA TTC TGG TG
		Acceptor probe	TGG TGT TAC TCA CGT TGT TCC
		NH forwardc	ACC GAA GCT CCA ATG AAT CCA AAA TCC
		NH reversec	GTT TGG TCA ATA CCA GCA GCT TCC AAA

^aDonor probes were labeled at the 3′ end with fluorescein.

^bAcceptor probes were labeled at the 5′ end with LightCyler Red 640.

^cACT, ERG11, and MDR1 Northern hybridization probe sequences were previously described (5).

^dLC, LightCycler; NH, Northern hybridization.