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Comparison of BLS1-BLS2 and EDTA reagents for bacteria permeabilization and labeling. S. epidermidis (a gram-positive organism) (a) and E. coli (a gram-negative organism) (b) were spiked at 103 and 104 CFU/ml, respectively, in water, PBS, TSB, or plasma. The samples were immediately processed with BLS1-BLS2 (□) or EDTA (▪) solution. Bacteria were detected and counted with the Scansystem analyzer. The numbers of bacteria obtained by both methods were compared.
