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. 2013 Dec 4;14(1):850. doi: 10.1186/1471-2164-14-850

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© Scully et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Histogram of transcript lengths generated from 454 FLX and Illumina paired end reads using Trinity. Co-assembly of 454 shotgun reads and Illumina paired-end sequences using Trinity yielded 42,085 transcripts (31 Mb) ranging in length from 200 to 32,701 nt with an N50 transcript length of 945 nt. This represented a substantial improvement over the 454 assembly and generated more full length transcripts in comparison.