FIG. 2.

Western blot analysis of Afmp1p and Afmp2p in sonicated fungal cell lysate and concentrated culture supernatant of A. fumigatus with guinea pig sera. A band of about 60 kDa was detected when serum from a guinea pig immunized with Afmp1p reacted with concentrated culture supernatant (lane 1). A band of about 45 kDa (probably due to core glycosylation) and a broad band of about 60 to 100 kDa (probably due to various forms of glycosylation) were detected when the serum reacted with sonicated fungal cell lysate (lane 2). A band of about 200 kDa was detected when serum from a guinea pig immunized with Afmp2p reacted with concentrated culture supernatant (lane 3), and a very faint band of the same size was detected when the serum reacted with sonicated fungal cell lysate (lane 4). No bands were detected with preimmune guinea pig serum (lanes 5 and 6).