FIG. 2.

PCR amplification using four sets of primers (3A, 3B-1, 3B-2, and 7C) derived from bacteriophage-like DNA using commercial Taq polymerase reagents from company A (A) and company B (B). PCR products were run on a 1.5% agarose gel with Tris-acetate-EDTA buffer. Lane 1, UV-treated double-distilled H₂O (ddH₂O; Gibco-Invitrogen) with cloned DNA 3A, 3B, or 7C for the respective primer sets tested; lane 2, UV-treated laboratory ddH₂O; lane 3, laboratory ddH₂O without UV treatment; lane 4, UV-treated ddH₂O (Gibco-Invitrogen); lane 5, ddH₂O (Gibco-Invitrogen) without UV treatment; lane 6, 100-bp DNA ladder size marker.