Table 8.
Expression patterns of select candidate genes regulating metabolism, development and immunity
| Function | Candidate genes | AM ID | Flybase ID | Control: Nosema | Nurse: Forager | Rich: Poor |
|---|---|---|---|---|---|---|
| Insulin signaling, metabolism and behavioral maturation | Insulin-like peptide 2 | AM00001 | FBgn0044050 | Control | Nurseǂ | Richǂ |
| PDK1 | AM08362 | FBgn0020386 | Nosema | Forager | NR | |
| Akt1 | AM08268 | FBgn0010379 | Control | NR | NR | |
| Foxo | AM06472, AM04383* | FBgn0038197 | Nosema | NR | Poor | |
| Lipase-1 | AM03164 | FBgn0032264 | Control | Nurse | Rich | |
| Lipid storage droplet 2 | AM11728 | FBgn0030608 | Control | Foragerǂ | NRǂ | |
| Cpt1 | AM11065 | FBgn0027842 | Control | Nurseǂ | Richǂ | |
| Usp | AM09226 | FBgn0003964 | Nosema | NR | NR | |
| Gigas | AM06808 | FBgn0005198 | Control | NR | NR | |
| Juvenile hormone epoxide hydrolase | AM03396 | FBgn0010053 | Control | Nurseǂ | Richǂ | |
| Juvenile hormone esterase | AM07915 | FBgn0010052 | Control | Nurseǂ | Richǂ | |
| Trehalose-6-phosphate synthase 1 | AM05412 | FBgn0027560 | Nosema | Forager | Rich | |
| Venus kinase receptor | AM08479 | No orthologue | Nosema | Forager | NR | |
| Immunity | ECSIT | AM11800 | FBgn0028436 | Control | NR | Rich |
| Pelle | AM08978 | FBgn0010441 | Control | Forager | Rich |
*AM04383 was only significantly regulated in this study.
ǂData from qRT-PCR [43].
Directional expression of candidate genes was compared between this study (control:co-infected workers, 14 days pi) and expression in nurses versus foragers and workers fed on rich versus poor diets obtained from microarrays or quantitative real-time PCR [43]. Treatment names in the table indicate upregulation of a given transcript in the assigned treatment group relative to the other experimental group. NR indicates that no significant regulation was identified between groups.