TABLE 3.
Sensitivity and specificity of PCR assays
| PCR using primer pair | Target | No. of PCR positive/no. tested
|
Sensitivity (%) | Specificity (%) | ||
|---|---|---|---|---|---|---|
| Pseudomonas sp.a | P. aeruginosab | Otherc | ||||
| PA GS-F and PA GS-R | Pseudomonas species | 28/28 | 14/14 | 0/43 | 100 | 100 |
| PA SS-F and PA SS-R | P. aeruginosa | 0/28 | 14/14 | 0/43 | 100 | 100 |
a
Culture collection Pseudomonas species other than P. aeruginosa (detailed in Table 1).
b
Culture collection P. aeruginosa only (detailed in Table 1).
c
Previously identified non-Pseudomonas species recovered from CF sputum (includes 15 B. cepacia complex, 5 Pandoraea sp., 5 Ralstonia sp., 5 A. xylosoxidans, 4 S. maltophilia, 2 Acinetobacter sp., and 2 S. marcescens strains and 1 strain each of H. frisingense, K. pneumoniae, M. morganii, M. osloensis, and E. coli).