Figure 1.

Evaluation of PBAEs for oncosphere transfection efficacy and safety. A wide screen of PBAE formulations, including different PBAE chemical structures and polymer/DNA ratios (w/w), was used to transfect 3-D BTIC JHGBM-551 oncospheres. All PBAEs used here (x-axis) were synthesized at a 1.2:1 B:S ratio. Leading polymers from this screen were chosen on the basis of high transfection efficacy (percent of cells transfected) and low toxicity (loss of relative metabolic activity). Fluorescence microscopy shows GFP⁺ oncospheres transfected with DsRed using purified PBAEs.