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. 2013 Nov 1;14(1):751. doi: 10.1186/1471-2164-14-751

Table 1.

Differentially expressed proteins identified by MALDI-TOF/TOF

Spot no. Protein name Organism NCBI accession no. Theoretical MW(kDa)/pl Fold change Database Mowse scorea No. of peptides EC no.
Proteins increased in diapause larval hemolymph
a1 Serpin 3a Manduca sexta gi|27733415 51.13/5.26 2.8 EST_Har 311 4 -
a5 Ommochrome binding protein M. sexta gi|400673 30.61/5.89 1.5 EST_Har 369 4 -
b6 Glutathione S-transferase Helicoverpa amigera gi|297340786 23.72/6.18 2.8 EST_Har 202 3 EC:2.5.1.18
b7 Hydroxypyruvate isomerase Danaus plexippus gi|357604475 29.12/6.00 specific EST_others 151 2 EC: 5.3.1.22
b8 Pyridoxine 5′-phosphate oxidase Bombyx mori gi|112984194 29.87/8.76 1.5 EST_Har 120 2 EC:1.4.3.5
c5 C-type lectin 7 H. armigera gi|385202653 34.99/5.98 2.3 EST_Har 116 2 -
c7 Serpin H. armigera gi|314912147 43.53/5.04 2.7 EST_Har 92 2 -
c9 Imaginal disc growth factor-like protein Mamestra brassicae gi|85726208 47.83/7.08 3.8 NCBI 99 1b EC:3.2.1.14
d4 Beta-1,3-glucan recognition protein 3 H. armigera gi|208972535 53.37/5.85 1.9 NCBI 262 4 EC:3.2.1-
d5 Putative serine protease-like protein 2 Lonomia obliqua gi|56462300 32.15/5.79 5.9 EST_others 182 2 EC:3.4.21.-
d6 C-type lectin 7 H. amigera gi|385202653 34.99/5.98 7.4 EST_Har 128 2 -
d7 C-type lectin 7 H. amigera gi|385202653 34.99/5.98 5.5 EST_Har 102 2 -
d8 Serpin H. amigera gi|314912147 43.53/5.04 2.9 EST_Har 165 2 -
d11 Ferritin M. sexta gi|7272336 26.51/5.44 2.6 EST_Har 193 3 -
Proteins decreased in diapause-destined larval hemolymph
a2 Growth-blocking peptide binding protein Heliosthis virescens gi|238915973 23.63/5.16 1.7 EST_others 79 1 -
a3 Sulfatase D. plexippus gi|357631807 56.65/5.34 2.2 EST_others 105 1 EC:3.1.6.14
a4 Sulfatase D. plexippus gi|357631807 56.65/5.34 2.3 EST_others 114 1 EC:3.1.6.14
a6 EndoU protein, partial Popilio polytes gi|389615052 28.70/8.38 3.3 EST_Har 217 4 EC:3.1--
a7 Kazal-type inhibotor D. plexippus gi|357625979 22.97/5.07 1.8 EST-Har 229 2 -
b1 Protein disulfide isomerase B. mori gi|112984454 55.59/4.60 5.7 EST_others 140 2 EC:5.3.4.1
b3 Ommochrome-binding protein M. sexta gi|400673 30.61/5.89 2.8 EST_Har 207 3 -
b5 Hemolymph proteinase 18 M. sexta gi|56418417 44.14/5.94 2.0 EST_Har 49 1 EC:3.4.21-
c2 Serine proteinase-like protein 1 H.armigera gi|208972549 46.51/5.43 1.8 NCBI 129 3 EC:3.4.21-
c3 C-type lectin H. armigera gi|106897087 38.01/5.42 1.9 EST_Har 49 1 -
c4 C-type lectin H. armigera gi|106897087 38.01/5.42 2.9 EST_Har 118 1 -
d1 Serine proteinase-like protein 1 H. armigera gi|208972549 46.51/5.43 10.7 NCBI 84 2 EC:3.4.21-
d2 Serine proteinase-like protein 1 H. armigera gi|208972549 46.51/5.43 10.7 NCBI 148 3 EC:3.4.21-
d12 Unknown secreted protein Papilio xuthus gi|389609461 17.50/8.55 2.3 EST_Har 195 3 EC:3.4.21

aMowse scores greater than the significance threshold indicate identity or extensive homology (p < 0.05). Three database have their respective significance thresholds, the EST_Har database is 31, the NCBInr database is 46, and the NCBI EST_others database is 57.bAdditional data for single peptide-based identifications are presented in Additional file 2: Figure S2.