Table 1.
Spot no. | Protein name | Organism | NCBI accession no. | Theoretical MW(kDa)/pl | Fold change | Database | Mowse scorea | No. of peptides | EC no. |
---|---|---|---|---|---|---|---|---|---|
Proteins increased in diapause larval hemolymph | |||||||||
a1 | Serpin 3a | Manduca sexta | gi|27733415 | 51.13/5.26 | 2.8 | EST_Har | 311 | 4 | - |
a5 | Ommochrome binding protein | M. sexta | gi|400673 | 30.61/5.89 | 1.5 | EST_Har | 369 | 4 | - |
b6 | Glutathione S-transferase | Helicoverpa amigera | gi|297340786 | 23.72/6.18 | 2.8 | EST_Har | 202 | 3 | EC:2.5.1.18 |
b7 | Hydroxypyruvate isomerase | Danaus plexippus | gi|357604475 | 29.12/6.00 | specific | EST_others | 151 | 2 | EC: 5.3.1.22 |
b8 | Pyridoxine 5′-phosphate oxidase | Bombyx mori | gi|112984194 | 29.87/8.76 | 1.5 | EST_Har | 120 | 2 | EC:1.4.3.5 |
c5 | C-type lectin 7 | H. armigera | gi|385202653 | 34.99/5.98 | 2.3 | EST_Har | 116 | 2 | - |
c7 | Serpin | H. armigera | gi|314912147 | 43.53/5.04 | 2.7 | EST_Har | 92 | 2 | - |
c9 | Imaginal disc growth factor-like protein | Mamestra brassicae | gi|85726208 | 47.83/7.08 | 3.8 | NCBI | 99 | 1b | EC:3.2.1.14 |
d4 | Beta-1,3-glucan recognition protein 3 | H. armigera | gi|208972535 | 53.37/5.85 | 1.9 | NCBI | 262 | 4 | EC:3.2.1- |
d5 | Putative serine protease-like protein 2 | Lonomia obliqua | gi|56462300 | 32.15/5.79 | 5.9 | EST_others | 182 | 2 | EC:3.4.21.- |
d6 | C-type lectin 7 | H. amigera | gi|385202653 | 34.99/5.98 | 7.4 | EST_Har | 128 | 2 | - |
d7 | C-type lectin 7 | H. amigera | gi|385202653 | 34.99/5.98 | 5.5 | EST_Har | 102 | 2 | - |
d8 | Serpin | H. amigera | gi|314912147 | 43.53/5.04 | 2.9 | EST_Har | 165 | 2 | - |
d11 | Ferritin | M. sexta | gi|7272336 | 26.51/5.44 | 2.6 | EST_Har | 193 | 3 | - |
Proteins decreased in diapause-destined larval hemolymph | |||||||||
a2 | Growth-blocking peptide binding protein | Heliosthis virescens | gi|238915973 | 23.63/5.16 | 1.7 | EST_others | 79 | 1 | - |
a3 | Sulfatase | D. plexippus | gi|357631807 | 56.65/5.34 | 2.2 | EST_others | 105 | 1 | EC:3.1.6.14 |
a4 | Sulfatase | D. plexippus | gi|357631807 | 56.65/5.34 | 2.3 | EST_others | 114 | 1 | EC:3.1.6.14 |
a6 | EndoU protein, partial | Popilio polytes | gi|389615052 | 28.70/8.38 | 3.3 | EST_Har | 217 | 4 | EC:3.1-- |
a7 | Kazal-type inhibotor | D. plexippus | gi|357625979 | 22.97/5.07 | 1.8 | EST-Har | 229 | 2 | - |
b1 | Protein disulfide isomerase | B. mori | gi|112984454 | 55.59/4.60 | 5.7 | EST_others | 140 | 2 | EC:5.3.4.1 |
b3 | Ommochrome-binding protein | M. sexta | gi|400673 | 30.61/5.89 | 2.8 | EST_Har | 207 | 3 | - |
b5 | Hemolymph proteinase 18 | M. sexta | gi|56418417 | 44.14/5.94 | 2.0 | EST_Har | 49 | 1 | EC:3.4.21- |
c2 | Serine proteinase-like protein 1 | H.armigera | gi|208972549 | 46.51/5.43 | 1.8 | NCBI | 129 | 3 | EC:3.4.21- |
c3 | C-type lectin | H. armigera | gi|106897087 | 38.01/5.42 | 1.9 | EST_Har | 49 | 1 | - |
c4 | C-type lectin | H. armigera | gi|106897087 | 38.01/5.42 | 2.9 | EST_Har | 118 | 1 | - |
d1 | Serine proteinase-like protein 1 | H. armigera | gi|208972549 | 46.51/5.43 | 10.7 | NCBI | 84 | 2 | EC:3.4.21- |
d2 | Serine proteinase-like protein 1 | H. armigera | gi|208972549 | 46.51/5.43 | 10.7 | NCBI | 148 | 3 | EC:3.4.21- |
d12 | Unknown secreted protein | Papilio xuthus | gi|389609461 | 17.50/8.55 | 2.3 | EST_Har | 195 | 3 | EC:3.4.21 |
aMowse scores greater than the significance threshold indicate identity or extensive homology (p < 0.05). Three database have their respective significance thresholds, the EST_Har database is 31, the NCBInr database is 46, and the NCBI EST_others database is 57.bAdditional data for single peptide-based identifications are presented in Additional file 2: Figure S2.