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. 2013 Nov 23;14(1):824. doi: 10.1186/1471-2164-14-824

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© Chang et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SUMO-2/3 enrichment in stabilizing gene transcriptional during KSHV reactivation. (A) TREx-F3H3-K-Rta-shSUMO-2/3 BCBL-1 cells were treated with Dox for 24 and 48 hours. TCLs were analyzed by immunoblotting using anti-SUMO-2/3 antibody. (B) Twelve IRF-1, IRF-2 and IRF-7 targeted genes showing SUMO-2/3 enrichment at the promoter region during KSHV reactivation were chosen. Two genes showing no SUMO-2/3 enrichment at the promoter region were chosen as control. RNA samples derived from TREx-F3H3-K-Rta BCBL-1 and TREx-F3H3-K-Rta-shSUMO-2/3 BCBL-1 cells before and after 24 hours of Dox induction were subjected to reverse transcription (RT) reaction. Following the RT reaction, the IRF target genes were amplified by qPCR using gene-specific primer sets. All reactions were run in triplicate and normalized against GAPDH.