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. 2013 Nov 23;14(1):824. doi: 10.1186/1471-2164-14-824

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© Chang et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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K-bZIP is able to SUMOylate IRF-1 and IRF-2 and to interact with IRF-7. Flag-tagged IRF-1 (A), IRF-2 (B) and IRF-7 (C) were transiently co-transfected with the indicated tagged constructs. Total cell lysates (TCLs) were prepared 48 hours after transfection. The lysates were immunoprecipitated with M2-beads and analyzed by immunoblotting using anti-IRF-1 (A; right panel), anti-IRF-2 (B; right panel), anti-SUMO-2/3 (A and B; left panel) and anti-IRF-7 (C) antibodies. (D) TCLs from TREx-F3H3-K-bZIP BCBL-1 cells before and after 48 hours of Dox induction for K-bZIP overexpression were used for immunoprecipitation with anti-K-bZIP antibody. The immunoprecipitates and TCLs were analyzed by immunoblot with antibodies as indicated.