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. 2013 Nov 23;14(1):823. doi: 10.1186/1471-2164-14-823

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© Xie et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gene similarity of unigenes against the nr database. (A) Effect of query sequence length on the percentage of significant matches. The cut-off value was set at 1.0E-5. The proportion of sequences with matches in the nr database at NCBI is greater among the assembled sequences with a greater length. (B) E-value distribution of the top BLAST hits for each unigene (E-value of 1.0E-5). (C) Similarity distribution of the best BLAST hits for each unigene. (D) Distribution of BLAST results by species is shown as the percentage of the total homologous sequences (with an E-value ≤1.0E-5). All plant proteins in the NCBI nr database were used for homology search and the best hit of each sequence was used for analysis.