Table 2.
Assembler | Data type | kmer | Approx. coverage | Avg identity | Indels < 5 bp | Indels > = 5 bp | Inversions | Unalignable contigs | Relocations | SNPs |
---|---|---|---|---|---|---|---|---|---|---|
Velvet | MiSeq | 59 | 100X | 99.99 | 13 | 38 | 2 | 0 | 16 | 42 |
59 | 75X | 99.99 | 23 | 31 | 0 | 0 | 12 | 110 | ||
59 | 50X | 99.99 | 18 | 36 | 1 | 0 | 17 | 88 | ||
59 | 25X | 99.99 | 36 | 129 | 0 | 0 | 8 | 96 | ||
Ray | MiSeq | 36 | 100X | 100 | 1 | 6 | 2 | 19 | 2 | 9 |
36 | 75X | 99.99 | 5 | 10 | 0 | 11 | 2 | 35 | ||
36 | 50X | 99.99 | 2 | 3 | 0 | 8 | 2 | 26 | ||
36 | 25X | 100 | 5 | 5 | 1 | 4 | 4 | 41 | ||
Ion | 29 | 100X | 99.86 | 5645 | 29 | 0 | 4 | 1 | 67 | |
29 | 75X | 99.9 | 4209 | 14 | 0 | 4 | 1 | 58 | ||
29 | 50X | 99.93 | 2784 | 7 | 0 | 0 | 0 | 58 | ||
29 | 25X | 99.95 | 1933 | 3 | 0 | 1 | 1 | 54 | ||
MIRA | MiSeq | n/a | 100X | 99.99 | 8 | 6 | 13 | 2 | 15 | 73 |
n/a | 75X | 99.99 | 20 | 4 | 11 | 0 | 11 | 124 | ||
n/a | 50X | 99.99 | 31 | 5 | 15 | 0 | 9 | 113 | ||
n/a | 25X | 99.99 | 9 | 7 | 7 | 0 | 7 | 81 | ||
Ion | n/a | 100X | 99.98 | 207 | 6 | 11 | 4 | 11 | 50 | |
n/a | 75X | 99.98 | 239 | 8 | 14 | 2 | 9 | 42 | ||
n/a | 50X | 99.98 | 292 | 10 | 9 | 1 | 8 | 58 | ||
n/a | 25X | 99.98 | 524 | 10 | 9 | 0 | 3 | 34 |
The bolded assemblies represent the best assembly for the specified combination of sequencer and software.