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. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Science. 2012 Dec 7;338(6112):1340–1343. doi: 10.1126/science.1226135

Figure 3.

Figure 3

Normalized UV-vis spectra of a selected group of hCRBPII mutants (M1-M11) are displayed. As indicated in the table, the absorption of the mutants spans from 425 nm (M1) to 644 nm (M11). For comparison, the wavelengths of various rhodopsins are included. The pigments in the cuvettes were generated by incubation of all-trans-retinal (72 µM in 100 mM phosphate buffer, pH 7.3, shown in the left cuvette) with hCRBPII mutants M1-M11 (80 µM protein, same concentration of retinal was used in all cuvettes).