Table I.
Origin and sequences of primers
| Primers | Sequences (5′ to 3′) | OrientationA | SourceB |
|---|---|---|---|
| ZMG120 | TATAGGATCC(-847)TAGTGATGGTCCTATCATATTCGTA | (sense) | HH0407B |
| BamHI | |||
| ZMG121 | TATAGTCGAC(84)AATACCTCGTTCTTTGCGA | (antisense) | HH0407B |
| HincII | |||
| ZMG122 | TATAGTCGAC(2418)TATGCTAGGAATTGTATTAATGTA | (sense) | HH0407B |
| HincII | |||
| ZMG123 | TATACTCGAG(2919)TCCCATAAGGATAAGCCACGCT | (antisense) | HH0407B |
| XhoI | |||
| ZMG174 | (262)GCCTATGGGTGGTATAGTTTATTTTGTT | (sense) | HH0409B |
| ZMG175 | (411)TCCCATAAGGATAAGCCACGCT | (antisense) | HH0409B |
| ZMG54 | TATAGTCGAC(-96)GTGATATAGATTGAAAAGTGG | (sense) | M35190C |
| HincII | |||
| ZMG55 | TATAGTCGAC(643)AGTGCGACAAACTGGGATT | (antisense) | M35190C |
| HincII |
The numbers in parentheses denote the 5′ nucleotide of each primer in reference to the start codon of the respective genes: -, upstream of a start codon; +, downstream of a start codon.
A
Orientation of the primer to the coding strand of each gene.
B
Sequences of these genes were published by Suerbaum et al. [16].
C
The sequence of the cat gene was published by Wang and Taylor [30].