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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 9;192(10):4610–4619. doi: 10.4049/jimmunol.1300692

Figure 3. Human markers validate the developmental status of B cell subsets identified by CD21 and CD24 co-expression.

Figure 3

A, Diagram of gating strategy to exclude both CD27+ and CD27− IgM+ memory B cells in human tissues. B-C, PB and BM cells were co-stained for flow cytometry to detect IgM, CD27, CD38, CD24, and CD21. IgM+CD27− cells in lymphocyte light scatter were gated as shown to identify memory, transitional and mature B cell subsets based CD24/CD38 expression as shown (left panel). Cells in the mature and transitional subsets were gated and their distribution with respect to CD21/CD24 subsets is shown (right panels). D, IgM+CD27− cells in the CD24/CD38 non-memory gate (dashed oval in panel A) were gated into CD21/CD24 subsets and evaluated for CD38, CD10 and CD5 expression. Data shown are representative of n=27 adult human PB and n=10 adult human BM.